The effect of melatonin on galectin-3, TNF-α, and IL-1βin newborn rats brain damage after hypoxia-ischemia and its impact on long-term behavior
10.3969/j.issn.1000-3606.2014.05.021
- VernacularTitle:褪黑素对新生大鼠缺氧缺血后脑中galectin-3、TNF-α、IL-1β水平及长期行为能力的影响
- Author:
Lili QIAO
;
Weiqin SHEN
- Publication Type:Journal Article
- Keywords:
melatonin;
hypoxic-ischemic brain damage;
long behavioral test;
rat
- From:
Journal of Clinical Pediatrics
2014;(5):480-484
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate neuroprotective effect of melatonin on preterm rats brain damage after hypoxia-ischemia (HI). Methods In this study, 5-day-old Wistar rats were divided into four groups: normal saline group, melatonin group, HI+NS group and HI+melatonin group. HI was conducted by unilateral ligation of the left common carotid artery (ische-mia) and 50 min of hypoxia. Melatonin was injected at a dose of 5 mg/kg intraperitoneally three times:before ischemia, after hy-poxia and 24 h after the second dose. The pups were sacrificed at 24 h, 72 h, and 7 weeks after HI;for galectin-3 cells count at 72 h and 7 weeks;TNF-α, IL-1βprotein were measured in 24 h and 72 h after HI;and fear condition and elevated plus maze were tested in 7 weeks after HI. Results The number of galectin-3-positive cells was lower after melatonin treatment than vehi-cle treatment in 72 h and 7 weeks after HI (all P<0.05). TNF-αprotein and IL-1βprotein both increased at 24 h and 72 h after HI, and reduced after melatonin treatment (all P<0.05). Melatonin treatment improved memory ability and learning ability, re-duced anxiety in 7 weeks after HI. Conclusions Melatonin has long-term and short-term protective effect on developing brain damage after HI.
