Detection of phenotype and analysis of genotype in vancomycin-resistant Enterococci
- VernacularTitle:耐万古霉素肠球菌耐药表型检测和基因型分析
- Author:
Xiajia ZHANG
;
Jilu SHEN
;
Weihua JIA
- Publication Type:Journal Article
- Keywords:
vancomycin;
drug resistance;
enterococci;
phenotype;
genotype
- From:
Acta Universitatis Medicinalis Anhui
2014;(5):662-664
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the resistant characteristics, genotype and prevalence of vancomycin-resistant Enterococci( VRE) . Methods K-B disc diffusion method was used to determine the susceptibility, minimum inhibi-tory concentration ( MIC) for vancomycin of VRE was detected by E-test method;VRE was then subjected to PCR for resistance related genes;6 strains sequencing results of PCR product were contrastively analyzed the amino acid sequence by BLAST. Results 6 VREFm strains were found from 193 strains enterococci;6 VREFm strains were completely resistant to high unit gentamicin, ampicillin, ciprofloxacin, teicoplanin,but were sensitive to linezolid and macrodantin,MIC for vancomycin was more than 256 mg/L;Genotypes were vanA;Amino acids in vanA gene have changed with Asn83→Asp( AAC→GAC) . Conclusion VRE in our hospital is mostly multi-drug resistant , which bringS difficulty in clinical treatment for its infection. So the hospital should strengthen the prevention and monitoring, to prevent the spread of VRE strains in the hospital and popular.
