Optimization of Macroporous Resin Purification Process for Total Flavonoids in Papaya
- VernacularTitle:木瓜总黄酮大孔树脂纯化工艺的优选
- Author:
Haijun QIN
;
Yi ZHANG
;
Ling MA
;
Shinong CHEN
;
Yanru REN
- Publication Type:Journal Article
- Keywords:
Macroporous resin;
Papaya;
Total flavonoids;
Separation and purification;
Content determination
- From:
China Pharmacist
2014;(4):550-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the macroporous resin separation process for total flavonoids in papaya. Methods:The content of total flavonoids in papaya was selected as the index, and the resin model, sample solution concentration, ratio of diameter and height, the flow rate of adsorption, type and volume of eluent, type and volume of impurity removing solvent, elution velocity and the other parameters were investigated. Results:The optimal purification process was as follows: the macroporous resin type was D-140, the sample solution concentration was 0. 1 g·ml-1 , the sample volume was 2BV, the ratio of diameter and height was 1∶9, washing the impurities with 3BV water, eluting with 3BV 10% ethanol first followed by 3BV 50% ethanol with 2BV·h-1 , and collecting 50%ethanol elution. The total flavonoids content was 52%. Conclusion:The optimized process can separate and purify the total flavonoids in papaya effectively.
