Determination of Baicalin and Polydatin in Kanggan Liyan Syrups by HPLC
- VernacularTitle:HPLC法同时测定抗感利咽糖浆中黄芩苷和虎杖苷的含量
- Author:
Liangyong HUANG
;
Youlin ZHANG
;
Jiangping ZHENG
- Publication Type:Journal Article
- Keywords:
HPLC;
Baicalin;
Polydatin;
Kanggan Liyan syrups;
Determination
- From:
China Pharmacist
2014;(4):703-705
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of baicalin and polydatin in Kanggan Liyan syrups. Methods:The samples were analyzed on an Waters SunFire C18 column with the mobile phase A of acetonitrile and the mobile phase B of 0. 2% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 284nm,and the column box temperature was 30℃. Results:Baicalin and polydatin could be separated effectively without interference. The linear range of baicalin was 32. 0-480. 0 μg·ml-1 and the average recovery was 98. 71%(RSD=0. 67%,n=5). The linear range of poly-datin was 16. 0-240. 0 μg·ml-1 and the average recovery was 97. 02%(RSD=1. 03%,n=5). Conclusion:The method is accurate and stable, and can be used in the determination of Kanggan Liyan syrups.
