Determination of spinosin in Anshenbao granule by HPLC
10.3760/cma.j.issn.1673-4246.2014.05.015
- VernacularTitle:高效液相色谱法测定安神宝颗粒中斯皮诺素的含量
- Author:
Zhengjun WANG
;
Ming LYU
- Publication Type:Journal Article
- Keywords:
Spinosin;
Anshenbao granule;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2014;(5):442-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of Spinosin in anshenbao granule. Methods The HPLC was adopted with Phenomenex LUNA-C18(4.6 mm×250 mm, 5μm)column, and the mobile phase consisted of Acetonitrile(19)-0.005 mol/L Kaliumdihydrogenphosphorate(81). The flow rate was 1.0 ml/min, the column temperature was 30℃ and the UV detector was set at 250 nm. Results The linear response range was 1.83~36.60μg/ml(r=0.9998). The average recovery of spinosin was 98.4%, and RSD1.22%. Conclusion The method was simple, rapid, accurate and repeatable. It can be applied in determination of spinosin in Anshenbao granule.
