The effect and mechanism of DADS inhibiting the proliferation and inducing apoptosis of gastro-esophageal adenocarcinoma cell line OE19
10.7652/jdyxb201403019
- VernacularTitle:DADS抑制食管胃交界部腺癌细胞OE 19增殖及诱导凋亡的机制
- Author:
Xiaoran YIN
;
Cheng FENG
;
Jun ZHANG
;
Hongbing MA
;
Xijing WANG
;
Shuqun ZHANG
;
Yang JIAO
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
DADS;
gastro-esophageal adenocarcinoma;
proliferation;
apoptosis;
OE1 9
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2014;(3):370-374
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of DADS in inhibiting the proliferation and inducing apoptosis of gastro-esophageal cancer cells in vitro.Methods The gastro-esophageal adenocarcinoma cells OE1 9 were treated by DADS of different concentrations in vitro.Morphologic changes were observed by the microscope and MTT assay was performed to test the growth-inhibitory effect of DADS on OE1 9 cells.Apoptosis rate of OE1 9 treated with different concentrations of DADS was measured by flow cytometry.Real-time PCR was used to detect DADS-induced effects on mRNA expressions of Caspase-3 ,Caspase-9 ,Bcl-2 ,Bax and NF-κB in OE1 9 cells.Results DADS inhibited the proliferation of OE19 cells in a dose-dependent manner.The apoptosis rate of OE19 cells was 14.0%,25.4% and 19.0% and 27.2%,respectively,when treated with 40 and 80μg/mL DADS for 24 h and 48 h.Real-time PCR assay showed that DADS could enhance mRNA expression levels of Caspase-3 and Caspase-9 and significantly decrease the mRNA expression levels of NF-κB and Bcl-2 and the ratio of Bcl-2/Bax. Conclusion DADS can significantly inhibit the proliferation and induce the apoptosis of gastro-esophageal adenocarcinoma cells via mitochondria-dependent pathways,which may be related to NF-κB and Bcl-2 families.
