Cystatin M inhibits the proliferation and migration of gastric cancer SGC-7901 cell lines
10.7652/jdyxb201403020
- VernacularTitle:Cystatin M抑制胃癌SGC-7901细胞的增殖和迁移
- Author:
Yamin ZHAO
;
Wei HE
;
Feng WANG
;
Guoqiang ZHAO
;
Yanwei YE
;
Qingxia FAN
- Publication Type:Journal Article
- Keywords:
CST6 gene;
cystatin M;
gastric cancer;
SGC-7901 cell;
proliferation;
migration
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2014;(3):375-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.
