Curcumin inhibits LPS-induced EMT through down-regulating NF-κB-Snail signaling in breast cancer cells
10.7652/jdyxb201403025
- VernacularTitle:姜黄素通过NF-κB-Snail信号通路抑制LPS诱导的乳腺癌细胞上皮间质化
- Author:
Hong JI
;
Guifang LU
;
Tao SHAN
;
Yiming LI
;
Hongwei LU
;
Jinjin SHANG
;
Tao HUANG
- Publication Type:Journal Article
- Keywords:
curcumin;
breast cancer;
Snail;
invasion;
epithelial-mesenchymal transition (EMT);
Vimentin;
E-cadherin;
NF-κB
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2014;(3):399-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether epithelial-mesenchymal transition (EMT)is involved in the anti-invasion and anti-metastasis effects of curcumin on MDA-MB-2 3 1 breast cancer cell line and further analyze the underlying mechanisms.Methods MTT method was used to detect the anti-proliferative effect of curcumin on MDA-MB-2 3 1 cell line induced by lipopolysaccharide (LPS).The morphological changes were determined by optical and transmission electron microscopy,respectively.The expressions of Vimentin,E-cadherin,NF-κB and Snail were analyzed using RT-PCR and Western blotting.Results Curcumin could inhibit the occurrence of LPS-induced EMT of MDA-MB-2 3 1 breast cancer cell line, decrease the expression of LPS-induced EMT marker Vimentin and increase the expression of E-cadherin, resulting in the inhibition of in vitro cell motility and invasiveness.These actions were mediated by inactivating NF-κB-Snail signaling pathways.Conclusion Our data provide a new perspective of the anti-invasion mechanism of curcumin,indicating that the effect is in part due to its ability to inhibit the EMT process.
