Effects of rhein lysinate on the expressions of TNF-α,IL-6 and NF-κB in the kidney tissue of SAMP 1 0 mice
10.7652/jdyxb201403027
- VernacularTitle:赖氨大黄酸对快速老化小鼠SAMP 10肾组织TNF-α、IL-6、NF-κB表达的影响
- Author:
Yongzhan ZHEN
;
Gang HU
;
Yufang ZHAO
;
Ran LI
;
Guangling ZHANG
;
Lihua ZHU
;
Yajun LIN
- Publication Type:Journal Article
- Keywords:
rhein lysinate;
senescence accelerated mouse prone 1 0 (SAMP 1 0 );
glomerulonephritis;
tumor necrosis factor-alpha (TNF-α);
IL-6;
NF-κB;
anti-aging
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2014;(3):411-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of rhein lysinate (RHL)on the expressions of TNF-α,IL-6 and NF-κB in the kidney tissue of senescence accelerated mouse prone 10 (SAMP 10)mice.Methods We selected 1 8 male mice (SAMP 1 0 )aged 7 months for the study and randomly divided them into blank control group and groups of different concentrations of RHL;six senescence accelerated mouse resistance 1 (SAMR 1 )served as the young control group.After 6 weeks’ treatment,HE staining was used to detect the pathological changes of the kidney.The expressions of TNF-α,IL-6 and NF-κB at the protein level were detected by immunohistochemistry and Western blotting.Results RHL treatment did not affect the body weight of SAMP 10 mice (P>0.05 ). Compared with SAMR 1 mice, contracted and destroyed renal glomeruli and infiltration of mononuclear macrophages were observed in control SAMP10 mice.However,this pathological process was blocked by RHL (25 mg/kg and 50 mg/kg ) treatments. In addition, the overexpressions of TNF-α, IL-6 and NF-κB and the phosphorylation of NF-κB in the kidney tissue of SAMP 10 mice could be inhibited by RHL treatments (P<0.05). Conclusion RHL inhibits the inflammatory reaction of the kidney tissue,which may be one of the mechanisms by which RHL exerts its kidney-protecting and anti-aging effects.
