β1-adrenergic receptor autoantibodies inhibit the proliferation of different subtypes of T lymphocytes in patients with heart failure
10.3760/cma.j.issn.0254-5101.2014.03.008
- VernacularTitle:心衰患者血清中β1肾上腺素受体自身抗体抑制不同亚型T淋巴细胞的增殖
- Author:
Xiao LI
;
Yunhui DU
;
Tingting LYU
;
Yulin GONG
;
Huirong LIU
;
Xinliang MA
- Publication Type:Journal Article
- Keywords:
Heart failure;
β1-adrenergic receptor;
Autoantibody;
CD4 +T lymphocyte;
CD8 +T lymphocyte
- From:
Chinese Journal of Microbiology and Immunology
2014;(3):205-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of β1-adrenergic receptor autoantibodies (β1-AA) on the proliferation of different subtypes of T lymphocytes in patients with heart failure .Methods β1-AA-positive IgG antibodies isolated from patients with heart failure were purified by using affinity chromatog -raphy.CD3 +CD4 +T and CD3 +CD8 +T lymphocytes were sorted by flow cytometry analysis .The prolifera-tion of different subtypes of T lymphocytes was tested by using CCK-8 kit.Tests for lactate dehydrogenase ( LDH) level and cell apoptosis were performed to evaluate T lymphocytes damage .Results The prolifera-tion of activated lymphocytes was inhibited by β1-AA isolated from the serum of patients with heart failure in a concentration dependent manner , but it could be blocked by β1 receptor blocker .The damage of lympho-cytes induced by β1-AA was increased.Moreover, β1-AA promoted the necrosis and apoptosis of CD 3 +CD4 +T and CD3 +CD8 +T lymphocytes and thus inhibited the proliferation of them .Conclusion β1-AA isolated from the serum of patients with heart failure inhibited the proliferation of CD 3 +CD4 +T and CD3 +CD8 +T lymphocytes through increasing the necrosis and apoptosis of them .This study suggests that β1-AA might induce immune disorders in addition to causing pathological changes in heart tissues .
