Changes of high mobility group box 1 expression in hepatocellular carcinoma tissues
- VernacularTitle:高迁移率族蛋白B1在肝细胞癌组织中的表达改变
- Author:
Feifei HAN
;
Tingting WANG
;
Guoqian CHEN
- Publication Type:Journal Article
- Keywords:
High mobility group box 1 protein;
Hepatoma;
Protein expression
- From:
Journal of Medical Postgraduates
2014;(4):344-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective High mobility group box 1 (HMGB1) mediates the inflammatory immune response as well as the prolif-eration, differentiation and migration of cells , neverthless, little study has been done on the changes of HMGB 1 expression in hepatoma tissues.The aim of the article was to investigate the changes of HMGB 1 mRNA and protein expression in hepatocellular carcinoma (HCC) tissues. Methods Real-time fluorescence quantitative PCR and Western blotting were applied to detect HMGB 1 mRNA and protein expression in HCC tissues and their paracancerous tissues of 13 cases.Immunofluorescence localization was used to analyze the changes of HMGB1 distribution in hepatic cells . Results The expression of HMGB1 mRNA and protein in HCC tissues (4.01 ±0.81) and (1.37 ±0.34) increased significantly in comparison with those in paracancerous tissues (5.76 ±1.28) and (0.52 ±0.12) ( P<0.01).Nucleo-cytoplasmic translocation of HMGB1 was observed in hepatocarcinoma cells . Conclusion There is increased expression of HMGB1 in HCC tissues, which shows HMGB1 mediates the carcinogenesis of HCC .
