Effects of gemcitabine on the ceil cycle and apoptosis of pancreatic cancer cells MiaPaCa-2 with Beclinl gene silencing
10.3760/cma.j.issn.1674-1935.2014.01.008
- VernacularTitle:吉西他滨对Beclinl基因沉默的人胰腺癌MiaPaCa-2细胞的周期及凋亡的影响
- Author:
Hongyu LI
;
Xiaoshu LI
;
Lisheng WANG
;
Yuefeng YANG
;
Xiaozhong GUO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
RNA interference;
Gene silencing;
Beclinl;
Gemcitabine;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Pancreatology
2014;14(2):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of gemcitabine on the cell cycle and apoptosis of the human pancreatic cancer cell line MiaPaCa-2 with Beclinl gene silencing.Methods siRNA targeting at Beclinl gene was constructed,then it was inserted into an expression vector and transfected into MiaPaCa-2 cells.The Beclinl mRNA and protein expression were detected by RT-PCR and Western blot.Gemcitabine was used to treat MiaPaCa-2 with Beclinl gene silencing,then the cell cycle and apoptosis were detected by flow cytometry.Results The MiaPaCa-2 cells with Beclinl gene silencing were successfully constructed,and the expression of Beclin1 mRNA was decreased from 1.0 in control group to 0.295,and number of cells in S and G2 phase was decreased,but number of cells in G1 phase was increased,and there was no change in apoptosis.After gemcitabine treatment,number of cells in S phase was further decreased,but number of cells in G1,G2 phase was increased,and apoptosis was inhibited.Conclusions Beclinl gene silencing can change the cell cycle of pancreatic cancer cells MiaPaCa-2,and influence the effects of gemcitabine on cell cycle and apoptosis.
