Expression of apoptotic protease cysteinyl aspartate specific proteinase-3 in cortex of rats with sepsis
10.3760/cma.j.issn.2095-428X.2014.08.019
- VernacularTitle:脓毒症大鼠大脑皮质含半胱氨酸的天冬氨酸蛋白水解酶-3表达的意义
- Author:
Yunjie SU
;
Xihong LI
;
Xin YANG
;
Jing WANG
;
Yi QU
;
Dezhi MU
- Publication Type:Journal Article
- Keywords:
Sepsis;
Brain injury;
Cortex;
Apoptosis;
Cysteinyl aspartate specific proteinase-3
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(8):632-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of apoptotic protease cysteinyl aspartate specific proteinase (caspase-3) in cortex neurons of rats with sepsis.Methods Models of rats with sepsis were established by the cecal ligation and puncture (CLP).Totally 70 cases of 30-day-old male Wistar rats were randomly divided into CLP group (n =50) and control group (n =20).In CLP group,CLP was performed in the rats.Neurobehavioral score was measured in 5 rats at 6,12,24 and 48 h after CLP surgery,respectively.Then,they were killed and their brains were removed.The immunohistochemical staining and Western blot were used to detect the apoptotic protein caspase-3 expression in cortex of rats.Control group did not undergo CLP,and the other treatment was the same as CLP group.Results Neurobehavioral scores at 12,24 and 48 h after CLP surgery were significantly lower than that in the control group(t =3.651,3.773,7.155,all P ＜ 0.05),and the scores were gradually decreased,overall situation of rats was getting worse along with the time.Caspase-3 protein expressed only in trace amounts in rat cerebral cortex in the control group by immunofluorescence analysis,however,its expression was significantly increased at 12 h after CLP surgery.Western blot test showed that caspase-3 protein expression in rat cerebral cortex at 6,12 and 24 h after CLP surgery was significantly higher than those in control group (all P ＜ 0.05).Its expression began to increase at 6 h after CLP surgery,and reached the peak at 12 h,then decreased at 48 h.Conclusion The neurobehavioral scores decreases and the expression of apeptosis protease caspase-3 increases in cortex of rats with sepsis brain injury.
