Determination of the contents of eugenol in Dijin spray by GC-MS
10.3760/cma.j.issn.1673-4246.2014.03.012
- VernacularTitle:气相色谱-质谱法测定地锦喷雾剂中丁香酚的含量
- Author:
Aiwu WANG
;
Qinghua ZHAO
;
Wei GONG
;
Jie MIAO
- Publication Type:Journal Article
- Keywords:
GC-MS;
Dijin spray;
Eugenol
- From:
International Journal of Traditional Chinese Medicine
2014;36(3):237-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the eugenol in Dijin spray by GC-MS.Methods The chromato-graphic column was DB-5 fused silica capillary column.Eugenol was determined by program temperature risen method:the initial temperature was 60 ℃ for 1 min,then raised to 200 ℃ at the rate of 10 ℃/min and kept for 5 min,and at last raised to 250 ℃ at 15 ℃/min and kept for 5 min.Electron energy was 70ev.The ions of 164,149,and 77 were selected for eugenol detection.Results The calibration curve of eugenol was linear in the range of0.116～1.16 mg/ml (r=0.999 4) with the average recovery of 97.84％ (RSD=0.47％).Conclusion The method was quick,accurate and repeatable.It was suitable for the quality control of Dijin spray.
