Effects of pulsed electromagnetic fields on homing genes of mouse osteoblasts

VernacularTitle:脉冲电磁场对小鼠成骨细胞归巢基因的作用
Author: Fujun YANG ; Yuanming SUN ; Wenqing XU ; Jie ZHAO ; Dezhi WANG ; Feiyue FAN
Publication Type:Journal Article
Keywords: Pulsed electromagnetic fields; Osteoblasts; Stem cell niche
From: International Journal of Biomedical Engineering 2014;37(2):81-84
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of pulsed electromagnetic fields (PEMF) on homing and proliferation-related genes of mouse osteoblasts.Methods 9 week-old C57BL/6 mice were treated with PEMF (70 Hz,1 mT) for 4～5 weeks,while mice in control group didn't not receive PEMF.Bone marrow cells of femurs and tibias were flushed out,and the bones were minced and incubated at 37 ℃ with a type Ⅰ collagenase.Bone associated mononuclear cells (MNCs) were isolated via density centrifugation with Lymphoprep.Magnetic cell sorting was used before flow-cytometric sorting,and the ALCAM+Sca-1-cells were collected.The homing and proliferation-related genes expressed in ALCAM+Sca-1-cells were detected with high throughput microarray and RT-PCR.Results The expression of Jag1 and Ang-1 in mouse osteoblasts increased under the effects of PEMF.Conclusions PEMF may have regulation effects on HSC (hematopoietic stem cell) survival through modulating the homing and proliferationrelated genes in ALCAM+Sca-1-osteoblasts.