The effect of TGF-β1, TGF-βRI and high concentration iodine in the promotion of fibroblast ;proliferation：correlation study
10.3969/j.issn.1008-794X.2014.05.016
- VernacularTitle:转化生长因子β1及其受体与高碘促成纤维细胞增殖作用的相关性研究
- Author:
Qianjin HUA
;
Maoheng ZU
;
Lin HU
;
Yinping ZHUANG
- Publication Type:Journal Article
- Keywords:
Budd-Chiari syndrome;
fibroblast;
iodine;
cytokine;
membranous obstruction
- From:
Journal of Interventional Radiology
2014;(5):431-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between transforming growth factor-β1 (TGF-β1), transforming growth factor-β receptor Ⅰ (TGF-βRⅠ) and high concentration iodine in promoting fibroblast proliferation so as to explore the pathogenesis of the membranous formation in Budd- Chiari syndrome. Methods The experiment included five groups: blank control group, solvent group, KI group, TGF-βRⅠinhibitor group (SD-208) and SD-208 plus KI combination group. ① Fibroblasts were cultured in high content of iodine and treated with TGF-βRI inhibitor then the fibroblast proliferation activity was determined by CCK-8 assy.②The protein expressions of TGF-β1 and TGF-βRⅠof fibroblasts in different concentrations of iodine (0, 250, 500, 1 000, 2 000 and 3 000 ug/L) were determined by Western-blot method. Results ①When the culture solution was of 1 000 ug/L iodine concentration, the cell proliferation rate of the SD-208 plus KI combination group (A:1.29 ± 0.41) was significantly higher than that of the control group (0.52 ± 0.10), but significantly lower than that of the KI group(1.70 ± 0.03) with P < 0.05. ② Fibroblast TGF-β1 protein relative expression levels in the groups with the iodine concentration of 1 000 ug/L and 2 000 ug/L were significantly higher than those of the other groups (P < 0.05). No significant difference in fibroblast TGF-βRⅠ protein relative expressions existed between each other groups (P > 0.05). Conclusion ①High concentration of iodine may promote the proliferation of fibroblasts through raising TGF - β1 protein expression. ② The proliferation of fibroblasts caused by high concentration of iodine may be related to the membranous formation in Budd-Chiari syndrome.
