High-risk HPV genotyping for cervical intraepithelial neoplasia grade 2 or worse:A comparison of single types and type combinations
10.3969/j.issn.1007-3969.2014.05.004
- VernacularTitle:高危型HPV亚型及亚型组合检测宫颈癌及高级别宫颈上皮内瘤变的比较研究
- Author:
Xiaochun WAN
;
Huijuan YANG
;
Xiaoyan ZHOU
;
Libing XIANG
;
Wentao YANG
;
Xu CAI
;
Yongming LU
;
Ying CHEN
;
Bo PING
- Publication Type:Journal Article
- Keywords:
Invasive uterine cervical carcinoma;
Cervical intraepithelial neoplasia;
Human papillomavirus;
Genotyping
- From:
China Oncology
2014;(5):342-348
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Risk of invasive cervical carcinoma (ICC) and its precancerous lesions following high risk human papillomavirus (hrHPV) infection may vary according to HPV types and geographic regions. Analyzing HPV-type distribution in cervical samples from local women aged 30 years and older, this study aimed to identify HPV types with higher risk of developing CIN2+, and to compare diagnostic performance for CIN2+using these types and type combinations. Methods:Cervical samples with histology follow-up from patients of a tertiary cancer center in Shanghai were collected for HPV genotyping by PCR-RDB. The risk associations of HPV types with CIN2+were estimated by logistic regression analysis, and ROC curves were plotted for diagnostic performance evaluation. Results:A total number of 413 specimens were obtained, including 38 CIN1, 184 CIN2/3, 126 ICC patients and 65 negative control people. The 4 most common HPV types in CIN2+were HPV16, 58, 33 and 18, in descending order. And only HPV16 (P<0.000 1), 58 (P=0.002), 33 (P=0.015) were signiifcantly associated with CIN2+lesions. Besides, the area under the ROC curve of the HPV16/18/33/58 test scored statistically higher than the HPV16/18 test did (P=0.006 6). Conclusion:A combined test of HPV16/18/33/58 may offer better performance for detecting CIN2+lesions in our geographic region.
