The detection and significance of AKAP12 methylation levels in peripheral blood in patients with lung cancer
10.3969/j.issn.1671-8348.2014.15.004
- VernacularTitle:肺癌患者外周血 AKAP12基因甲基化水平的检测及意义
- Author:
Hehe LIAO
;
Yunfeng ZHANG
;
Shuqiang WU
;
Hanchun LI
;
Lei JIN
;
Hong REN
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
A-kinase anchoring protein 12;
methylation;
MS-HRM;
peripheral blood
- From:
Chongqing Medicine
2014;(15):1834-1836
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the levels of AKAP12 methylation by methylation‐specific high‐resolution melting curve(MS‐HRM ) in peripheral blood in patients with colorectal cancer and investigate its clinical significance .Methods We used MS‐HRM technology to detect the levels of AKAP12 methylation in peripheral blood in 60 lung cancer patients ,and analyzed the relationship between the levels of AKAP12 methylation and pathological parameters of lung cancer patients .Results 34(56 .7% ) of the 60 lung cancer patients were found to be methylated at the AKAP12 promoter region by MS‐HRM ,the methylation levels of 18 cases ranged between 1% -20% ,14 cases ranged between 20% -60% ,2 cases ranged between 60% -100% .There was no significant differences between the levels of AKAP12 methylation and lung cancer patients′age and gender(P>0 .05) .However ,it was signifi‐cantly higher in the patients with high pathological stage and differentiation degree (P<0 .05) .Conclusion AKAP12 promoter re‐gion methylation was related to tumor progression and malignant degree .
