Study on application of electrochemical microsensor detecting NO released from macrophage stimulated by Escherichia coli
10.3969/j.issn.1671-8348.2014.15.007
- VernacularTitle:电化学微传感器实时检测大肠埃希菌激活的RAW264．7细胞释放NO的研究
- Author:
Wangze WU
;
Jilu TANG
;
Tian GAN
;
Zhongxin LU
;
Zhi QIAO
- Publication Type:Journal Article
- Keywords:
nitric oxide;
macrophage;
escherichia coli;
electrochemical microsensor
- From:
Chongqing Medicine
2014;(15):1843-1845
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply nitric oxide(NO) electrochemical microsensor in the real time detection of NO released from RAW 264 .3 cells infected by E .coli ,and to explore the application value of this NO microsensor in the research area of infection im‐munity against bacterium .Methods Taking NO microsensor to detect NO released from RAW 264 .3 cells respectively stimulated by E .coli of different densities and of 1 × 107 mL -1 for different time .Results The level of NO released from RAW 264 .3 cells was enhanced obviously when incubated with E .coli as compared with that of normal cells and the extent of incersase depended on the density of E .coli (P<0 .01) .The released level of NO increased gradually from the beginning and reached its peal at the time of 12 h then decreased slowly when incubated with E .coli of 1 × 107 mL -1 .Conclusion The electrochemical microsensor was applied in the real time detection of NO released from macrophages activated by E .coli successfully .
