Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis.
- Author:
Won Seok CHOI
1
;
Kyung Hwan RYU
;
You Jeong KIM
;
So Young KIM
;
Hyun Hee KIM
;
Wonbae LEE
Author Information
1. Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. lwb@hfh.cuk.ac.kr
- Publication Type:Original Article
- Keywords:
Granulocyte-colony stimulating factor(G-CSF);
Granulocyte macrophage-colony stimulating factor(GM-CSF);
G-CSF receptor;
GM-CSF receptor;
Leukocytosis
- MeSH:
Antibodies, Monoclonal;
Child*;
Communicable Diseases;
Cytokines;
Granulocyte Colony-Stimulating Factor*;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Granulocytes*;
Humans;
Leukocytes;
Leukocytosis*;
Neutrophils;
Plasma*;
Receptors, Cell Surface;
Receptors, Granulocyte Colony-Stimulating Factor;
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
- From:Journal of the Korean Pediatric Society
2003;46(3):271-276
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Granulocyte-colony stimulating factor(G-CSF) and granulocyte macrophage-colony stimulating factor(GM-CSF) are principal cytokines in granulopoiesis and their physiologic effects are mediated through binding to specific cell surface receptors. Although it is known that the level of serum G-CSF and GM-CSF, and presentation of the receptors are increased in infectious diseases, there have been no studies to find the correlation between the granulopoiesis and leukocytosis. This study was designed to measure G-CSF and GM-CSF in leukocytosis and in control and to demonstrate the possible pathogenesis of granulopoiesis in leukocytosis using quantitative analysis of G- CSF, GM-CSF and their CSFr. METHODS: The plasma levels of G-CSF, GM-CSF of 13 children without leukocytosis and 14 children with leukocytosis were measured. Counts of cell surface G-CSFr and GM-CSFr were measured by combining anti G-CSFr and anti GM-CSFr monoclonal antibodies to their respective receptors by using quantitative flow cytometric assay. RESULTS: There was no significant difference betweeen the plasma concentration of G-CSF and GM-CSF in acute leukocytosis and in the control group. However, levels of G-CSFr in acute leukocytosis decreased significantly compared to the control(P=0.012) and the levels of GM-CSFr in both groups revealed no significant difference. CONCLUSION: Increase in the number of leukocyte in leukocytosis was mediated by increasing the number of neutrophil, and increased plasma concentration of G-CSF may be the cause of neutrophilia. But GM-CSF did not have any influence on leukocytosis.
