To constructe,package and identificate the lentiviral vector with overexpression gene Grp78
10.3969/j.issn.1671-8348.2014.15.028
- VernacularTitle:Grp78基因过表达慢病毒载体构建和包装及鉴定
- Author:
Yawen LI
;
Shiyuan XU
;
Qingguo ZHANG
;
Le LI
;
Luying LAI
;
Ting ZHENG
;
Jiaoling SU
;
Naimei YANG
;
Yuantao LI
- Publication Type:Journal Article
- Keywords:
Grp78 gene;
lentiviral vector;
gene engineering
- From:
Chongqing Medicine
2014;(15):1904-1906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To constructe ,package and identificate the lentiviral vector with overexpression gene Grp78 .Methods We used lentiviral vector and genetic engineering technology to obtain the aim gene fragment and to constructe recombinant plas‐mid .we prepared competent cells and transform the cells .Through positive clone sequencing ,lentivirus was packaged and virus titer was tested .Results Positive cloning sequence comparison results show that the test was passed .Melt curve did not appear mixed peak ,also did not appear abnormal peak broadening .It means that does not appear pollution ,primer dimers and nonspecific amplifi‐cation in the experiments .Conclusion The construction ,packaging and identification of lentiviral vector with over expression gene Grp78 are sucessful .
