Effect of JPHSKL on the quantity of 5-Hydroxytryptamine of colonic mucosa of diarrhea-predominant irritable bowel syndrome rats
10.3969/j.issn.1671-8348.2014.14.011
- VernacularTitle:健脾化湿颗粒对实验性IBS-D大鼠结肠黏膜5-HT表达的影响
- Author:
Libo ZHAO
;
Yinghan WANG
;
Xiaofeng ZHANG
;
Hongjie GUO
- Publication Type:Journal Article
- Keywords:
irritable bowel syndrome;
serotonin;
Jian Pi Hua Shi Ke Li,5-HT
- From:
Chongqing Medicine
2014;(14):1713-1715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Jian Pi Hua Shi Ke Li(JPHSKL) on IBS‐D ,gastrointestinal peptide hormones of rats 5‐HT ,and explore the therapeutic mechanism .Methods 60 clean‐level Wistar rats were used in the experiment .The rats were randomly divided into normal group ,model group ,JPHS low ,middle ,high and Deshute group(10 rats in each group) .Used gavage senna and restraint stress to establish D‐IBS rat model .The drug contrations of JPHSKL low ,midle ,high group were 1 .5 ,2 .5 ,4 .0 g/mL ,3 mg/mol in Deshute group .Once each day ,for 2 weeks ,the other groups were nomol group without any treatment and mod‐el group which building successful .Immunohistochemical SABC method to observe 5‐HT level of colonic specimens .Results The 5‐HT level in colonic tissue of the rats in IBS‐D model were obviously higher than the nomal group(P<0 .01) ,and the 5‐HT level in colonic tissue in JPHSKL treated group were obviously lower than the mold group (P<0 .05) .Among them ,the JPHSKL high group had significant effect and there was no obviously difference with Deshute group (P>0 .05) .Conclusion JPHSKL can im‐prove gastrointestinal function of IBS‐D model rats ,effect mechanism is relating to decrease the level of 5‐HT in colonic mucosa .
