oxidized low-density-lipoprotein inhabits the proliferation and Oct-4 express of rat bone marrow mesenchymal stem cells in vitro
10.3969/j.issn.1671-8348.2014.14.020
- VernacularTitle:ox-LDL 抑制大鼠骨髓间充质干细胞的体外增殖和Oct-4表达
- Author:
Tiewei LV
;
Huichao SUN
;
Lei ZHANG
;
Lingjuan LIU
;
Xiaoyun WU
;
Xiaoyan LIU
;
Jing ZHU
;
Zhenguo LIU
;
Jie TIAN
- Publication Type:Journal Article
- Keywords:
lipoproteins,LDL;
bone marrow cells;
cell proliferation;
Oct-4
- From:
Chongqing Medicine
2014;(14):1737-1740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effect of ox‐LDL on self‐renewal and Oct‐4 express of MSCs in vitro .Methods MSCs cul‐tured in vitro were divided into 4 groups :blank control(no reagents in culture system ) ,ox‐LDL (1 ,5 ,10 ,20 μg/mL ox‐LDL were added into culture system) ,ox‐LDL+NAC(corresponding ox‐LDL were added into culture system after NAC treatment ) ,negative control(corresponding nLDL were added into culture system ) .Growth curve were drawn at different time ,Oct‐4 ,a stem cell special marker ,were detected by real‐time PCR ,the production of ROS (reactive oxygen species ,ROS) in culture system were measured with electron paramagnetic resonance spectroscopy .Results proliferation of MSCs was inhibited by ox‐LDL ,when concentration of ox‐LDL was more than 5μg/mL ;apoptosis of MSCs appeared as well as attenuated expression of Bcl‐2 ,ox‐LDL generated a signifi‐cant amount of ROS in the culture system ,which was completely prevented by NAC .Conclusion The proliferation and Oct‐4 ex‐pression of MSCs were inhibited by ox‐LDL ,which may be related to increase of ROS in culture system .
