Effects of Vasoactive Intestinal Peptide on Transforming Growth Factor-β1 Expression in Lung Tissue of Asthmatic Mice with Airway Remodeling
- VernacularTitle:血管活性肠肽对哮喘气道重塑小鼠肺组织转化生长因子β1表达的影响
- Author:
Juan WANG
;
Yunxiao SHANG
- Publication Type:Journal Article
- Keywords:
vasoactive intestinal peptide;
bronchial asthma;
airway remodeling;
transforming growth factor-β1
- From:
Journal of China Medical University
2014;(5):422-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of vasoactive intestinal peptide on transforming growth factor-β1(TGF-β1)expression in lung tis-sue of asthmatic mice with airway remodeling. Methods A total of 30 SPF grade female Balb/c mice were randomly divided into three groups:con-trol(group A),asthmatic(group B),and vasoactive intestinal peptide treatment(group C). Aerosolized ovalbumin was used to establish the asth-matic mice model. Pathological changes of bronchial and lung tissues were observed by HE,the perimeter of basement membrane(Pbm),internal wall area(WAi)and smooth muscle area(WAm)in bronchial lung tissue were analyzed using the pathological image. The expression levels of TGF-β1 mRNA and protein in lung tissue were detected by real-time PCR and Western blot. Results Pathologic changes including collagen deposition,in-filtration of inflammatory cells and smooth muscle layer thickness were found in group B. In addition,the WAi/Pbm and WAm/Pbm were significant-ly higher in group B than those of the group A(P<0.05),and lower in group C than in group B(P<0.05). The expression levels of TGF-β1mRNA and protein in group B were higher than both group A(P<0.05)and group C(P<0.05). Conclusion Vasoactive intestinal peptide may inhibit airway remodeling,which may be associated with reduced expression of TGF-β1.
