Genetic Polymorphisms of Glutathione S-transferase M1 and T1 and Evaluation of Oxidative Stress in Patients with Non-small Cell Lung Cancer
- VernacularTitle:谷胱甘肽S-转移酶M1、T1基因多态性及氧化应激与非小细胞肺癌易感性
- Author:
Hongyan ZHANG
;
Xuwei WU
;
Yi XIAO
;
Mei CHEN
;
Zhidong LI
;
Yanli LI
;
Kaifa TANG
- Publication Type:Journal Article
- Keywords:
glutathione S-transferases;
polymorphism;
oxidative stress;
non-small lung cancer
- From:
Journal of China Medical University
2014;(5):432-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes(GSTM1 and GSTT1),and eval-uate the oxidative damage in patients with non-small lung cancer(N-SCLC). Methods A total of 110 patients with N-SCLC and 100 healthy indi-viduals were recruited in this case-control study. Multiplex polymerase chain reaction(PCR)analysis was used to identify the genotypes. The activi-ty of malondialdehyde(MDA),nitric oxide(NO),and the total antioxidant capacity(T-AOC)were detected by spectroscopic analysis using assay kits. Results The frequencies of the GSTM1,T1,and GSTM1/T1 null genotypes in the patient group were significantly higher than those in control group(OR1=2.071,P1=0.009;OR2=1.900,P2=0.024;OR3=3.258,P3=0.003). The activity of MDA and NO were obviously higher in the pa-tient group compared with the control group(P<0.001),and T-AOC was obviously lower in patient group than those in control group(P<0.001). The activity of MDA,and NO were higher but the T-AOC were lower in patients with GSTM1,T1 and GSTM1/T1 null genotypes than those in pa-tients with GSTM1,T1 and GSTM1/T1 present genotypes(P<0.001). Conclusion Our results suggest that oxidative damage may play a impor-tant role in patients with N-SCLC,and the N-SCLC patients with GSTM1and GSTT1deletion genotypes are more susceptible to oxidative damage.
