Identification of Chemical Component Cantharidin from Chinese Blister Beetle by LC-MS/MS

VernacularTitle:LC-MS/MS研究斑蝥中斑蝥素类化学成分
Author: Yaobo ZENG ; Yuyu ZHANG ; Anming TANG ; Dajian YANG ; Chen LI ; Guoqiang LIU ; Yi ZHANG
Publication Type:Journal Article
Keywords: Chinese blister beetle; cantharidin; LC-MS/MS; qualitative analysis
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(4):876-882
CountryChina
Language:Chinese
Abstract: This study was aimed to verify the chemical component cantharidin from Chinese blister beetle by LC-MS/MS. Components were first processed by HPLC to choose the appropriate separation conditions before LC/MS/MS analysis. Through the positive and negative ions pre-scan, positive ions scanning way was selected in the scanning of each separate component. The scanned maps were obtained and the relative molecular mass was deduced. The results showed that 8 types of cantharidin compounds were identified, including three isomers. It was concluded that LC-MS/MS was able to determine the chemical component cantharidin from Chinese blister beetle rapidly and accurately, which was existed in the body by the form of combined amino acid.