HPLC Determination of Chlorogenic Acid, Rutin and Kaempferide in Solidago decurrens Lour
10.11842/wst.2014.04.041
- VernacularTitle:HPLC法同时测定一枝黄花药材中绿原酸、芦丁和山奈素的含量
- Author:
Hua FENG
;
Minghua NIE
;
Xiangpei WANG
;
Xiuqiong LUO
;
Xiaodong WANG
- Publication Type:Journal Article
- Keywords:
Solidago decurrens Lour;
chlorogenic acid;
rutin;
kaempferide;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(4):904-907
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an HPLC method for the determination of chlorogenic acid, rutin and kaempferide in Solidago decurrens Lour. A Diamonsil C18 column (4.6 mm í 200 mm, 5 μm) was adopted with the mobile phase of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL·min-1. The column temperature was at 25℃. The wavelength of detection was set at 282 nm. The results showed that the calibration curve was linear over the range of 63.2~442.4 μg for chlorogenic acid (r = 0.999 3), 8.1~56.8 μg for rutin (r = 0.999 4) and 10.8~75.7μg for kaempferide (r = 0.999 8). The average recovery of chlorogenic acid was 98.6% (RSD = 1.4%), that of rutin was 99.2% (RSD = 0.8%) and that of kaempferide was 100.3% (RSD = 1.0%). It was concluded that the method was simple, economical and accurate with good reproducibility.