HPLC Determination of Chlorogenic Acid, Rutin and Kaempferide in Solidago decurrens Lour

VernacularTitle:HPLC法同时测定一枝黄花药材中绿原酸、芦丁和山奈素的含量
Author: Hua FENG ; Minghua NIE ; Xiangpei WANG ; Xiuqiong LUO ; Xiaodong WANG
Publication Type:Journal Article
Keywords: Solidago decurrens Lour; chlorogenic acid; rutin; kaempferide; HPLC
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(4):904-907
CountryChina
Language:Chinese
Abstract: This study was aimed to establish an HPLC method for the determination of chlorogenic acid, rutin and kaempferide in Solidago decurrens Lour. A Diamonsil C18 column (4.6 mm í 200 mm, 5 μm) was adopted with the mobile phase of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL·min-1. The column temperature was at 25℃. The wavelength of detection was set at 282 nm. The results showed that the calibration curve was linear over the range of 63.2~442.4 μg for chlorogenic acid (r = 0.999 3), 8.1~56.8 μg for rutin (r = 0.999 4) and 10.8~75.7μg for kaempferide (r = 0.999 8). The average recovery of chlorogenic acid was 98.6% (RSD = 1.4%), that of rutin was 99.2% (RSD = 0.8%) and that of kaempferide was 100.3% (RSD = 1.0%). It was concluded that the method was simple, economical and accurate with good reproducibility.