Study on the inlfuening faltors of altivity detemination of four coagulation factors in human prothrombin complex concentrates
- VernacularTitle:人凝血酶原复合物中凝血因子活性测定的影响因素研究
- Author:
Tian TIAN
;
Haijun CAO
;
Yao CAO
;
Zechao HE
;
Changqing LI
- Publication Type:Journal Article
- Keywords:
PCC;
protamine sulfate;
dilution buffer;
salt concentration;
standard human plasma
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(2):17-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inlfuence factors on detection of activity of four coagulation factors in prothrombin complex concentrates (PCC) by several factors. Methods Using Pharmacopoeia of the People’s Republic of China (2010) as reference, the activity of four coagulation factors in PCC were investigated by choosing different pre-treatments, different diluents, different salt concentration, different standard human plasma and different company reagents. Results The activity of FII, FVII, FX were decreased and FIX was increased in the condition of adding protamine sulfate, and there were no differences of four coagulation factors whether warm bath in 37 for 15 min or not. However, the differences of four coagulation factors were significant by using deficient plasma, saline, distilled water and commercial dilution buffer(P<0.05). The activity of coagulation factor II, X in 1 mol/L salt concentration of PCC were significantly lower than in 0.25 mol/L(P<0.05), while coagulation factor VII, IX were not. The activity of FII, FVII, FIX, and FX were different by using different standard human plasma to make standard curve. The activity of four coagulation factors existed significant difference(P=0.00) by using SIEMENS company reagents and domestic reagents. Conclusion Choosing different pre-treatments, different dilution buffers, salt concentration, standard human plasma and commercial kits will inlfuence the detection result of coagulation factors.
