Comparison of Sperm Chromatin Structure Assay with Sperm Chromatin Dispersion Test for DNA Fragmentation Evaluation in Human Sperm
10.3969/j.issn.1006-5725.2014.05.048
- VernacularTitle:精子染色质结构分析和染色质扩散试验检测精子DNA碎片的比较研究
- Author:
Wuhua NI
;
Jianyuan JIN
;
Xu YANG
;
Qianjin FEI
;
Chengshuang PAN
;
Xuefeng HUANG
- Publication Type:Journal Article
- Keywords:
Sperm DNA fragmentation;
Sperm Chromatin Structure Assay (SCSA);
Sperm chromatin dispersion test (SCD)
- From:
The Journal of Practical Medicine
2014;(5):821-823
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD) for DNA fragmentation evaluation in human infertility, and the correlation between these two methods. Methods We used SCSA and SCD assays to detect DNA fragmentation in sperm from 134 infertile men. The correlation of SCSA and SCD assays was analyzed. The sperm DNA fragmentation index (DFI) was divided into 3 groups (≤15%DFI, >15~≤30%DFI and>30%DFI), and the difference between SCSA and SCD assays was assessed. Results The SCSA assay was strongly correlated with the SCD assay for sperm DNA fragmentation (r=0.915, P<0.001). There was no significant difference between>15~ ≤30%DFI and>30%DFI groups. However, SCD showed higher levels of DNA fragmentation than that measured by SCSA for≤15%DFI group (13.50 4.82 vs 9.79 2.60, P<0.001). Conclusion There is a strong positive correlation between SCSA and SCD assays in detection of DNA fragmentation. SCD assay showed higher levels of DNA fragmentation than that measured by SCSA for≤15%DFI group.
