Study on Quality Standard for Lianqiaobaidu Pills
10.3969/j.issn.1005-5304.2014.05.029
- VernacularTitle:连翘败毒丸质量标准研究
- Author:
Lin WU
;
Guang YANG
;
Fei HUANG
- Publication Type:Journal Article
- Keywords:
Lianqiaobaidu Pills;
TLC;
HPLC;
chlorogenic acid
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(5):95-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To imporve the quality standard of Lianqiaobaidu Pills. Methods The TLC method was established for ephedrae herba, sophorae flavescentis radix, paeoniae radix rubra, and RP-HPLC methed was established for the determination of chlorogenic acid in Lianqiaobaidu Pills. Zorbax SB-C18 (4.6 mm×150 mm, 5 μm) column was used with methanol-acetonitrile (2∶1) - 0.04% phosphoric acid (10∶90) as the mobile phase at flow rate of 0.8 mL/min. The detection wavelength was 326 nm. Results TLC chromatographic spots were clear, and negative control had no interference. The linear range of chlorogenic acid was 0.042 2-0.464 2 μg (r=1), the average recovery (n=6) was 98.96%. Conclusion The method is simple, reliable, with strong exclusive feature, and suitable for quality control of Lianqiaobaidu Pills.
