Simultaneous Determination of Three Main Contents in Scrophulariae Radix Dispensing Particles by HPLC-UV Dual Wavelength Spectrometry Method
10.3969/j.issn.1005-5304.2014.05.024
- VernacularTitle:高效液相色谱-紫外双波长法同时测定玄参配方颗粒中3种主要成分的含量
- Author:
Xiaoya ZHOU
;
Feie LAI
;
Xuemei LIU
- Publication Type:Journal Article
- Keywords:
Scrophulariae Radix dispensing particles;
harpagide;
harpagoside;
cinnamic acid;
HPLC-UV dual wavelength spectrometry
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(5):78-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a simultaneous HPLC-UV dual wavelength spectrometry method for detecting three main contents - harpagide, harpagoside and cinnamic acid in Scrophulariae Radix dispensing particles. Methods Ultimate AQ-C18 column (250 mm×4.6 mm, 5μm) was used, with 1%acetic acid solution and acetonitrile as mobile phase of gradient elution. The flow rate was 1.0 mL/min, detection wavelength was 210 nm at former 13 min and 278 nm after 13 min. The column temperature was 30 ℃. Results The linear range of harpagide, harpagoside and cinnamic acid was 0.066 54-0.665 4 μg (r=1.000 0), 0.024 23-0.242 3 μg (r=0.999 9), and 0.100 28-1.002 8 μg (r=0.999 9), respectively. The average recovery (n=6) was 99.80%±1.22%, 100.31%±1.30% and 100.22%±1.24%, respectively. Conclusion The method is simple, repeatable, stable, and can be used for quality control and standardization of Scrophulariae Radix dispensing particles.
