Preparation and Determination of Encapsulation Efficiency and Content of Bererine Hydrochloride Lipo-somes
- VernacularTitle:盐酸小檗碱脂质体的制备及其含量、包封率的测定
- Author:
Dengshan ZHANG
;
Liucheng LIU
- Publication Type:Journal Article
- Keywords:
Bererine hydrochloride liposomes;
Preparation;
Ammonium sulfate gradient method;
Encapsulation efficiency;
HPLC-ELSD;
Component content
- From:
China Pharmacist
2014;(4):601-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare bererine hydrochloride ( BER) liposomes and establish an effective method for the determination of content and entrapment efficiency. Methods:BER liposomes were prepared by ammonium sulfate gradient method. The encapsula-tion efficiency of BER liposomes was respectively determined by supercentrifugation method, microcolumn gel method and ultrafiltration method, and the content of every component in BER liposomes was detected by HPLC-ELSD. Results:The results showed that super-centrifugation method could precisely separate the free drug from the liposomes. The optimum parameters of supercentrifugation method were the centrifugal speed of 60 000 r·min-1 , the centrifugal time of 1 h, the centrifugal temperature of 10℃ and the lipid concentra-tion of 6 mg·ml-1 . Conclusion:The method is simple and sensitive with good separation efficiency. HPLC-ELSD can be used to de-termine the content of every component in BER liposomes.
