Study on Tissue Culture Techniques for Radicle of Bletilla Striata
- VernacularTitle:白及幼根组织培养技术研究
- Author:
Xiang LEI
;
Mengyao HUANG
;
Yanxia CHANG
;
Yuanyuan JING
;
Keli CHEN
- Publication Type:Journal Article
- Keywords:
Bletilla striata;
Tissue culture;
Rapid propagation
- From:
China Pharmacist
2014;(4):613-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To seek the best part and optimal culture medium for the tissue culture of Bletilla striata. Methods: The effects of hormone concentration and proportion on the induced differentiation, propagation and rootage of Bletilla striata were investiga-ted. Results:The radicle of Bletilla striata was the best part to induce the clustered shoots with the optimal culture medium of 1/2MS+1. 0 mg·L-1 6-BA+2. 0 mg·L-1 NAA. The best hormone concentration for inducing the clustered shoots was MS+1. 0 mg·-1 L 6-BA+0. 05 mg·L-1 NAA, and the optimal rooting medium was 1/2MS+0. 5 mg·L-1 NAA. Conclusion: The tissue culture system for Bletilla striata is established.
