The application value of HPV E6/E7 mRNA detection in paraffin-embedded samples for cervical cancer screening
10.3760/cma.j.issn.1006-9801.2014.04.009
- VernacularTitle:子宫颈石蜡包埋样本检测HPV E6/E7mRNA用于子宫颈癌筛查的价值
- Author:
Xing ZHOU
;
Jinku ZHANG
;
Wenming ZHAO
;
Xue CHEN
;
Bingjuan ZHOU
;
Hong CHEN
;
Jinmei LI
- Publication Type:Journal Article
- Keywords:
Human papillomavirus;
Uterine cervical neoplasms;
Cervical intraepithelial neoplasia;
RNA,messenger;
Paraffin embedding
- From:
Cancer Research and Clinic
2014;26(4):243-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application value of detecting HPV E6/E7 mRNA in cervical cancer screening and prognosis.Methods bDNA technology was used to detect the HPV E6/E7 mRNA levels in 301 cases of paraffin-embedded cervical samples that were grouped based on pathological diagnosis.Statistic differece between multiple groups were assessed by chi-square segmentation method.Results The positive detection rate of HPV E6/E7 mRNA is higher in cervical cancer group than that in CIN1-3 groups and cervicitis group,which were 94.29 ％ (66/70),30.30 ％ (20/66),50.00 ％ (19/38),59.46 ％ (44/74),5.66 ％ (3/50),respectively (all P ＜ 0.004 5).The HPV E6/E7-positive rates in all three CIN groups were higher than that in cervicitis group (P ＜ 0.004 5).The positive rates in CIN2 and CIN3 group were higher than that in CIN1 group (P ＜ 0.004 5).HPV E6/E7 mRNA levels in cervical cancer group were higher than those in CIN groups and cervicitis group (16 508.730±8 741.402,929:337±460.880,3 239.681±1 447.399,5 286.224±2 933.445,4.941±2.263,respectively,all P ＜ 0.05).CIN groups had higher copy numbers of HPV E6/E7 mRNA than cervicitis group (P ＜ 0.05).More copies of HPV E6/E7 mRNA were detected in CIN3 group than those in CIN1 group (P ＜ 0.05).Conclusions The positive rate and expression levels of HPV E6/ E7 mRNA was related to the severity of cervical diseases.HPV E6/E7 mRNA detection in paraffin-embedded samples have a high clinical application value in predicting the risk of cervical cancer.It' s an effective measure for cervical cancer screening and follow-up.
