The influence of bortezomib on migration and invasion in cultured human prostatic cancer cells and its mechanism

VernacularTitle:硼替佐米体外对前列腺癌细胞迁移和侵袭的影响及其机制
Author: Wei HU ; Wei WANG ; Zhenyu GAO
Publication Type:Journal Article
Keywords: Prostatic neoplasms; Bortezomib; Cell movement; Neoplasm invasiveness; Focal adhesion kinase
From: Cancer Research and Clinic 2014;26(2):98-101
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of bortezomib on cellular migration and invasion ability in cuhured human prostatic cancer cells DU145 and its mechanism.Methods Transwell method was used to detect cell migration.Invasion was assayed by Matrigel-coated invasion chambers.Expressions of FAK and Tyr397 proteins were analyzed by Western blot.Results After treated by bortezomib at the concentration of 10,20 nmol/L for 24 h,the invasion index of DU145 cells were (69.05±10.56) and (52.55±6.98),they were gradually reduced compared with untreated group (81.55±10.56) (P ＜ 0.05).The migration index were (39.35±6.45),(32.05±4.22),they were also reduced compared with untreated group (58.75±5.41) (P ＜ 0.05).The group of treated by bortezomib showed Tyr397 protein expression had been suppressed.However FAK protein had not marked change.Conclusions FAK is involved in the regulation of cellular migration and invasion function.Bortezomib might inhibit cells migration and invasion function by down regulation of Tyr397 expression.