In vitro biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cells
10.3969/j.issn.2095-4344.2014.12.021
- VernacularTitle:膨体聚四氟乙烯与人脂肪干细胞的体外生物相容性
- Author:
Liu YANG
;
Nan JIANG
;
Yangyang XU
;
Menglin ZHU
;
Jing CAO
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
polytetrafluoroethylene;
stem cells;
materials testing
- From:
Chinese Journal of Tissue Engineering Research
2014;(12):1932-1937
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Expanded polytetrafluoroethylene is a kind of porous polymer materials which is commonly used as clinical implants, and it has good biocompatibility, and is not easy to deformation or metamorphism. There is no existence of inflammation absorption reaction, and it al ows the cel migration and tissue ingrowth.
OBJECTIVE:To study the biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cel s.
METHODS:The passage 4 human adipose-derived stem cel s were co-cultured with expanded polytetrafluoroethylene scaffold in vitro. The morphology and function of cel s adhered to the scaffold were observed by inverted phase contrast microscope, and cel adhesive rates and proliferation rates were also calculated by MTT assay.
RESULTS AND CONCLUSION:The inoculated cel s were round and bright, distributed on the surface of scaffolds uniformly, with good cel viability. After 3 hours a large number of adherent cel s were observed from the micrograph;after 24 hours there were a smal amount of short-spindle adipose-derived stem cel s. After cultured for 3 days, the short fusiform or polygon cel s could be seen clearly. After cultured for 7 days, the number of cel s increased significantly, few cel s fel off from the scaffold, and cel adhesion rate was up to an average of 95.7%. Meanwhile, the cel s revealed normal splitting proliferation rate. These findings indicate that human adipose-derived stem cel s are able to attach, grow and proliferate wel on the scaffold. Expanded polytetrafluoroethylene reveals excel ent cel ular compatibility and can be used as a vehicle for adipose tissue engineering.
