Influence of recombinant hirudin on survival of rat dorsal avulsed flap
10.3760/cma.j.issn.1671-0290.2014.02.014
- VernacularTitle:重组水蛭素对大鼠撕脱皮瓣存活的影响
- Author:
Xiangyun ZHANG
;
Yi LIAO
- Publication Type:Journal Article
- Keywords:
Recombinant hirudin;
Avulsed flaps;
P-selectin;
Vascular endothelial growth factor (VEGF)
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2014;20(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of recombinant hirudin on avulsed flap and to explore the application of recombinant hirudin in clinical treating avulsed flap.Methods 108 Sprague-Dawley rats were randomly divided into three groups:local injecting recombinant hirudin (group A),local injecting low molecular heparin (group B),and local injecting physiological saline (group C).The far ends were sutured after forming dorsal avulsed flap with local freeing.The levels of P-selectin and VEGF were detected,and the inflammatory cell aggregation and formation of microthrombus were observed in pathological section.On 14th postsurgery day the survival rate of flap was calculated.Results The content of P-selectin in group A was lower than that of groups B and C,with a statistical difference (P＜0.05).The content of VEFG in group A was higher than that of groups B and C,with a statistical difference (P＜0.05).On 14th postsurgery day,group A was higher than that in groups B (P＜0.05)and C (P＜0.01) in the survival rate of flap.Conclusions Locally injected recombinant hirudin can actively suppress the production of P-selectin,reduce accumulation of inflammatory cells,prevent information of microthrombus,improve ischemia and anoxia of avulsed flaps,decrease injuries of endotheliocyte,enhance the expression of VEGF,and stimulate hyperplasia of neogenesis capillaries that elevate survival rate of avulsed flaps.
