Exogenous VEGF165 regulates skeletal myoblasts transplantation induced Th17/Treg imbalance
10.3760/cma.j.issn.1001-4497.2014.04.015
- VernacularTitle:外源性VEGF165调节骨骼肌成肌细胞移植诱导的Th17/Treg失衡
- Author:
Ruijun LIU
;
Changfa GUO
;
Chang CHEN
;
Gening JIANG
- Publication Type:Journal Article
- Keywords:
Myoblasts,skeletal;
Cell transplantation;
Vascular endothelial growth factors
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2014;30(4):242-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether Th17/Treg imbalance existed,and whether VEGF165 attenuated the imbalance in allogeneic skeletal myoblasts transplantation(allo-SMT) for acute myocardial infarction (AMI).Methods There were 60 C57BL/6 male mice,3 to 4 weeks old,weighting 0.016-0.020 kg.On the day 1,2,4,and 7 after allo-SMT,the percentages of Th17 and Treg cells,and the ratios of Th17/Treg cells were analyzed by FCM in AMI group,AMI-S group(alloSMT) and AMI-Ⅴ group(with VEGF165 treatment).Subsequently,related proinflammatory and regulatory cytokines and key transcription factors ROR-γt mRNA and Foxp3 mRNA expression were examined by Bio-plex and RT-PCR respectively.Results On the day 1,2,4,and 7 after allo-SMT,the percentage of Treg,related cytokines concentrations and transcript factor Foxp3 mRNA in AMI-S group were lower than those in AMI group,while the AMI-Ⅴ group were higher than those in AMI groups.However,the percentage of Th17 cells,related cytokines concentrations,and ROR-γt mRNA in AMI-S group were higher than those in AMI group; but the AMI-Ⅴ group were lower than those in AMI group.Apparently,compared with AMI group,the ratios of Th17/Treg significantly ascended in AMI-S group and decended in AMI-Ⅴ group.Conclusion Th17/Treg imbalance participated in the formation and development of inflammation and immune response after allo-SMT.However,transfected VEGF165 could relieve the severity of Th17/Treg imbalance.
