Co-culturing cytokine-induced killer cells with dendritic cells enhances the cytotoxic activities of CIKs against hepatocarcinoma cells
10.3760/cma.j.issn.0254-5101.2014.01.010
- VernacularTitle:DC与CIK共培养细胞体外抗肝癌细胞活性研究
- Author:
Ying WANG
;
Deping WU
;
Pingjing MING
;
Feilan YANG
;
Yu LIU
;
Shengjun LIU
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Cytokine-induced killer cells;
Cell phenotype;
Cytokine;
Hepatocarcinoma cells
- From:
Chinese Journal of Microbiology and Immunology
2014;34(1):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore in vitro cytotoxic activities of DCIKs against hepatocarcinoma cells by co-culturing cytokine-induced killer cells (CIKs) with dendritic cells (DCs) derived from peripheral blood of patients with hepatocellular carcinoma (HCC).Methods Peripheral blood mononuclear cells (PBMCs) were isolated from 23 patients with HCC and cultured with cytokines to induce DCs and CIKs.DCIKs were induced by co-culturing CIKs with DCs.After 14 days of co-culture,the phenotypes of DCIKs and CIKs were analyzed by flow cytometry,and their in vitro cytotoxic activities against SMCC-7721 and HepG2 hepatocarcinoma cells were measured by MTT assay.Levels of IL-12,IL-4 and IFN-γ in the supernatants of cell culture were detected by enzyme-linked immunosorbent assay (ELISA).Results High expressions of CD3+CD8+ and CD3+CD56+ were observed on DCIKs.The percentages of effector cells,cytotoxic activity and cytokine secretion were all significantly increased with DCIKs as compared with those CIKs without DC co-culture (P＜0.05).Conclusion Co-culture of CIKs with DCs can enhance the differentiation of effector cells and the cytolytic activities of CIKs against hepatocarcinoma cells in vitro.Immunotherapy with DCIKs may be a promising strategy for the treatment of patients with HCC.
