Mutation analysis of hepatitis B virus X gene in hepatitis B virus-associated glomerulonephritis
10.3760/cma.j.issn.1001-7097.2014.04.006
- VernacularTitle:乙型肝炎病毒相关性肾小球肾炎患者病毒X基因变异分析
- Author:
Hui DONG
;
Yan XU
;
Liqiu LIU
;
Guangju GUAN
;
Wei JIANG
- Publication Type:Journal Article
- Keywords:
Nephritis;
Hepatitis B virus;
Genes;
Mutation;
X gene
- From:
Chinese Journal of Nephrology
2014;30(4):273-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether mutation of Hepatitis B virus (HBV) X gene is associated with hepatitis B virus-associated glomerulonephritis (HBV-GN).Methods The venous blood was collected from 50 patients with HBV-GN and 60 patients with asymptomatic HBV carriers (control group).Serum HBV DNA was extracted to determine the serum titer of HBV-DNA and then polymerase chain reaction (PCR) was used to detect the HBV X gene mutation.Results (1)There were not statistical significance between age and gender in HBV-GN group and control group (P ＞0.05).There were not statistical significance of serum replication level of HBV DNA in HBV-GN with X gene mutation and control group (P ＞ 0.05).Urine protein excretion in HBV-GN group with or without X gene mutation was found with statistical significance (P ＜ 0.05).(2)Nucleotide mutations [84％ (42/50)] resulted in amino acid substitution in HBV-GN.Nucleotide mutations changed in transfunction control region of X gene,including position nt1653,nt1726,nt1727,nt1730,nt1753,nt1762 and nt1764.(3)Nucleotide mutations [8％(5/60)] resulted in amino acid substitution in control group.Nucleotide mutations changed in position nt1632 and nt1635,located in non-functional region.Conclusions HBV X gene mutations and the subsequent amino acid substitutions are found in HBV-GN.The urine protein excretion level increases in patients with X mutation,suggesting that these mutations may play an important role in the pathogenesis of HBV-GN.
