Pretreatment of SS31 prevents hypoxia/reoxygenation-induced apoptosis in renal tubular epithelial cells
10.3760/cma.j.issn.0254-1785.2014.04.010
- VernacularTitle:SS31对大鼠肾小管上皮细胞缺氧复氧损伤的影响作用及其机制
- Author:
Liang ZHU
;
Wenyu ZHAO
;
Li ZENG
;
Lei ZHANG
;
Fanyuan ZHU
;
Youhua ZHU
- Publication Type:Journal Article
- Keywords:
Anoxia;
Reperfusion;
Kidney tubular;
Epithelial cells;
In vitro;
Rats
- From:
Chinese Journal of Organ Transplantation
2014;35(4):232-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the pretreatment of SS31 could alleviate hypoxia/reoxygenation (H/R)-induced injury by inhibiting p66Shc.Method The cultured rat renal proximal tubular cell line NRK52E cells were exposed to 24-h hypoxia (5％ CO2,1％ O2,and 94％ N2) followed by 6-h reoxygenation (5％ CO2,21％ O2,and 74％ N2).SS31 was added to the culture medium 4 h prior to the treatment.Then the cell viability,apoptosis,ROS and MTP were determined.In addition,Western blotting was used to detect the expression of p66Shc and p-p66Shc.Result H/R induced apoptotic cell death,accompanied with activation of total and p-p66Shc in NRK52E cells.Total p66Shc and p-p66Shc were detected at low levels in control NRK52E cells,and their levels were dramatically increased in cells after H/R treatment.Pretreatment with 100 μmol/L SS31 significantly prevented cell death and attenuated total p66Shc and p-p66Shc levels after H/R.Conclusion This study revealed that SS31 pretreatment serves a protective role against H/R-induced apoptosis of human renal tubular epithelial cells by suppressing p66Shc.
