Correlation analysis of Treg cells and TGF-β1, IL-10 levels in a rat experimental liver carcinoma model
10.3760/cma.j.issn.1007-631X.2014.05.017
- VernacularTitle:大鼠实验性肝癌组织中Treg细胞与TGF-β1、IL-10水平的相关性分析
- Author:
Zhiqiang ZHANG
;
Jiefeng HE
;
Yu ZHANG
;
Zhiwei LAN
;
Haoliang ZHAO
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Interleukin-10;
Transformation growth factor beta 1;
Treg cells
- From:
Chinese Journal of General Surgery
2014;29(5):382-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between Treg cell numbers and level of TGF-β1,IL-10 in the immune microenvironment of rat liver cancer.Method 40 male Wistar rats were randomly divided into three groups,liver cancer model group (n =20),saline control group (n =10) and blank control group (n =10).Liver cancer model was established by intraperitoneal injection of DEN (100 mg/kg).Percentage of Treg cells in cancer tissues was detected by fiow cytometry and the expression level of TGF-β1 and IL-10 by immunohistochemical SABC.Results The ratio of Treg cells in CD4 + T cells was 14.32％ ± 4.84％ in liver cancer tissues,significantly higher than that in the blank control group 5.64％ ±6.10％ and the saline control group 7.95％ ±3.55％.The difference was statistically significant (F =211.279,P ＜ 0.05).The expression level of TGF-β1 and IL-10 in cancer tissues was significantly higher than the blank control group and the saline control group (FTGF-β1 =250.740,FIL-10 =152.744,all P ＜0.05).The number of Treg cells was positively correlated with TGF-β1,IL-10 level (P ＜ 0.05).Conclusions TGF-β1,IL-10 and Treg cells significantly increased in rat experimental liver cancer tissues,and there was positive correlation between Treg cells,and TGF-β1 and IL-10 in liver cancer tissues.
