The effects of TSH on human vascular endothelial cells and smooth muscle cells
10.3760/cma.j.issn.1000-6699.2014.03.016
- VernacularTitle:TSH对血管内皮细胞和平滑肌细胞影响的研究
- Author:
Limin TIAN
;
Jing NI
;
Jing LIU
;
Luyan ZHANG
- Publication Type:Journal Article
- Keywords:
Thyroid stimulating hormone;
Vascular endothelial cells;
Vascular smooth muscle cells;
Atherosclerosis
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(3):238-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of thyroid-stimulating hormone (TSH) on human vascular endothelial cells and smooth muscle cells and to explore the roles of TSH in the development of atherosclerosis.Methods Human vascular endothelial cells and smooth muscle cells were cultured in vitro.MTT method was used to assay the effect of TSH on cell viability.Real-time PCR was used to determine the levels of endothelial nitric oxide synthase (eNOS),prostacyclin(PGI2),endothelin-1 (ET-1),plasminagen activator inhibitor(PAI-1) (mRNA) in endothelial cells and the phenotype transition of smooth muscle cells.The effect of TSH to the cycle of smooth muscle cells was detected by using flow cytometry.Immunocytochemistry and Western blot were used to investigate the expression of the cell cyclin A,D1,and the expression of endothelial cell associated factors eNOS and ET-1.Results Compared with the control group,eNOS and PGI2 mRNA levels decreased while ET-1 and PAI-1 mRNA levels increased when different concentrations of TSH were applied to endothelial cells(P＜0.05).The level of eNOS protein was decreased gradually while the level of ET-1 protein was gradually increased(P＜0.05).Different concentrations of TSH applied to smooth muscle cells could promote the transition of cell cycle phase G2 to phase M and increase the expression of cell cyclin A and D1.Conclusion TSH may damage the function of vascular endothelial cells and promote the proliferation of smooth muscle cells.
