Effects ofβ1 adrenergic receptor autoantibodies on the micro-mechanics of a rat myocardial cell and the concentration of intracellular calcium
10.3760/cma.j.issn.0254-5101.2014.04.008
- VernacularTitle:β1肾上腺素能受体自身抗体对大鼠心肌细胞微区力及细胞内钙离子变化的影响
- Author:
Ruibing NIU
;
Jiantao FENG
;
Lin JIN
;
Cunbao LI
- Publication Type:Journal Article
- Keywords:
Heart disease;
β1-AR autoantibody;
Laser scanning confocal microscopy;
Atomic force microscopy
- From:Chinese Journal of Microbiology and Immunology
2014;(4):294-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of β1 adrenergic receptor (β1-AR) autoantibodies on the micro-mechanics of a single rat ventricular myocyte and the concentration of intracellular calcium for investigating the pathogenic mechanism of heart disease caused by β1-AR autoantibody on both cellular and molecular levels .Methods The micro-mechanics of an acutely isolated single myocardial cell from rat was detected by using the atomic force microscopy ( AFM) in combination with laser scanning confocal micro-scope (LSCM) before and after binding to β1-AR autoantibodies.The ventricular myocyte contraction and the intracellular calcium concentration were observed as well .Results The micro-mechanics of a single ventricular myocyte was increased from (44-51) nN to (76-82) nN after binding to β1-AR autoantibodies. Its contraction frequency was also increased from (0.17±0.04) Hz to (0.40±0.03) Hz (P<0.05).More-over, the intracellular calcium fluorescence intensity was increased significantly during contraction in com -parison with that before binding to β1-AR autoantibodies [ ( 102.1 ±12.3 ) % vs ( 154.3 ±16.7 ) %, P<0.01 ] .Conclusion β1-AR autoantibody could affect the contraction and the micro-mechanics of ventricu-lar myocytes and the intracellular calcium concentration in ventricular myocytes .
