α7nAChR is involved in anti-inflammation of physiological concentrations of glucocorticoids
10.3969/j.issn.1000-4718.2014.04.023
- VernacularTitle:α7 nAChR 参与生理浓度糖皮质激素的抗炎作用
- Author:
Yiyu CHEN
;
Mei ZHANG
- Publication Type:Journal Article
- Keywords:
Glucocorticoids;
α7 nicotinic acetylcholine receptor;
Microglia;
Lipopolysaccharides
- From:
Chinese Journal of Pathophysiology
2014;33(4):706-710
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the role of α7 nicotinic acetylcholine receptor (α7nAChR) in anti-inflammation of glucocorticoids (GCs) at physiological concentrations .METHODS: MTT assay was used to measure the viability of BV-2 cells, which were processed by hydrocortisone at different concentrations .On the basis of inflammatory model induced by LPS in BV-2 cells, experimental groups were divided as follows:(1) control;(2) LPS;(3) GCs+LPS;(4) methyl-lycaconitine ( MLA)+GCs+LPS.The levels of TNF-αand IL-1βin the cell supernatants were detected by ELISA .RE-SULTS:Hydrocortisone at concentrations of 2 000 and 1 000 nmol/L decreased the cell viability to (76.9 ±5.5)% and (90.8 ±7.3)%, respectively, indicating the cellular injury by GCs at over-physiological doses.LPS significantly induced the releases of TNF-αand IL-1βin a time-and dose-dependent manner in BV-2 cells.Hydrocortisone at physiological con-centrations (500 and 250 nmol/L) reduced the releases of TNF-αand IL-1βin BV-2 cells stimulated by LPS, and MLA at concentration of 10 nmol/L antagonized the anti-inflammatory effect of GCs .CONCLUSION:α7nAChR is involved in the anti-inflammatory effect of the physiological concentrations of GCs .
