Effects of endogenous histamine on transient forebrain ischemia-induced neuronal damage at late phase of reperfusion in mice
10.3969/j.issn.1000-4718.2014.04.003
- VernacularTitle:内源性组胺减轻小鼠前脑缺血再灌注后期脑损伤
- Author:
Yanying FAN
;
Yunpeng MA
;
Yuan QIAO
;
Ping HE
;
Xuanping ZHANG
;
Ohtsu HIROSHI
;
Zhong CHEN
- Publication Type:Journal Article
- Keywords:
Forebrain ischemia;
Neuronal injury;
Endogenous histamine
- From:
Chinese Journal of Pathophysiology
2014;33(4):592-597
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the effect of endogenous histamine on transient forebrain ischemia-induced neuronal injury at the late phase of reperfusion using histidine decarboxylase knockout ( HDC-KO) mice.METHODS:Wild-type (WT) and HDC-KO mice were subjected to bilateral common carotid artery occlusion for 30 min followed by 3 d or 15 d of reperfusion.At different time points after reperfusion, the body weight, mortality rate, learning and memory in fear conditioning test and hippocampal CA 1 neuronal density were evaluated .RESULTS: At 1 d after reperfusion , the body weight loss was observed in both WT and HDC-KO mice.At 4 d, 5 d, 6 d and 7 d after reperfusion, the increment in the body weight of the HDC-KO mice was significantly smaller than that of the WT mice .During the period between 8 d and 14 d after reperfusion, the mortality rate of the HDC-KO mice was higher than that of the WT mice (P<0.05).At 14 d after reperfusion , the HDC-KO mice exhibited more aggravated deficits in contextual and cue memory compared with the WT mice.Correspondingly , a more severe CA1 neuronal injury in the HDC-KO mice than that in the WT mice was ob-served at 15 d but not at 3 d after reperfusion (P<0.05).CONCLUSION:Endogenous histamine may attenuate learn-ing/memory deficits and neuronal injury at the late phase of ischemia /reperfusion.However, the involved mechanisms need to be further investigated .
