Central role of GRP78 in growth of gastric carcinoma cells

VernacularTitle:GRP78在胃癌生长中的作用
Author: Zhengqi FU ; Hongyan ZHEN ; Lijiang LIU
Publication Type:Journal Article
Keywords: Stomach neoplasms; Glucose-regulated protein 78; Cyclin D1
From: Chinese Journal of Pathophysiology 2014;33(4):625-628
CountryChina
Language:Chinese
Abstract: AIM:To explore the effect of glucose-regulated protein 78 (GRP78) on the gastric carcinogenesis. METHODS:GPR78 expression patterns were examined in 34 specimens from gastric carcinoma patients using the immu-nohistochemistry (IHC) assay, and in 10 specimens using Western blotting analysis .In addition, the expression of GPR78 and cyclin D1 was detected in human gastric cancer cell lines SGC 7901 and SGC7901-H78 (overexpressing GRP78) by Western blotting.RESULTS:By IHC assay, GRP78 was found to be highly expressed in the cytoplasm of gastric carcino-mas as compared with the adjacent non-malignant tissues and corresponding normal tissues .GRP78 expression was positive-ly correlated with gender and histological differentiation (P<0.05), but not with age, tumor stage and lymph node metas-tasis (P>0.05).Furthermore, we found that with the increased expression of GRP 78 in SGC7901-H78 cells, the expres-sion of cyclin D1 was also elevated .CONCLUSION:GRP78 might be a key player to be involved in the growth of gastric cancer.