Application of modified three-step fluorescence in situ hybridization in prenatal diagnosis
10.3760/cma.j.issn.1009-9158.2014.04.007
- VernacularTitle:改良荧光原位杂交三步法在产前诊断绒毛标本中的应用
- Author:
Yan ZENG
;
Jiaming FAN
;
Lifang ZHANG
;
Feiyan QIAN
;
Ping XU
;
Weijun JIANG
- Publication Type:Journal Article
- Keywords:
In situ hybridization,fluorescence;
Prenatal diagnosis
- From:
Chinese Journal of Laboratory Medicine
2014;37(4):266-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a simple and efficient fluorescence in situ hybridization method for prenatal diagnosis.Methods Thirty-six cases of chorionic villus samples were hybridized with probes 18/X/Y,13/21 by using traditional cuhure methods and modified three-step methods in controlled experimentation during 2012 to 2013.The hybridization rate and fluorescence signals were analyzed.Results A total of 72 hybrid zone were detected.Probe 21/13 hybridization rate and fluorescence signals of three-step modified methods were higher than that of traditional methods(99.72％ ±0.42％ vs 85.90％ ±4.15％,t =20.4,P ＜0.01; 2.58 ±0.50 vs 1.52 ± 0.55,t =7.53,P ＜0.01).Probe 18/X/Y has the same hybridization rate and fluorescence signals between three-step modified methods and traditional methods (99.57％ ±0.53％ vs99.70％ ±0.42％,t=1.30,P＞0.05; 2.22±0.42 vs2.36±0.48,t=1.57,P＞0.05).The coincidence rate of two methods was 100％ (36/36).Conclusion The modified fluorescence in situ hybridization three-step methods in the study was simple,rapid,effective and environment-friendly.At the same time,it has some defects such as the signals of 18/X/Y was not concentrated enough.Further exploration is needed.
