Effects of resveratrol on high glucose memory-induced injury to endothelial cell
10.3760/cma.j.issn.1000-6699.2014.05.017
- VernacularTitle:白藜芦醇对高糖诱导内皮细胞损伤代谢记忆效应的研究
- Author:
Hui DING
;
Peng WANG
;
Yangang WANG
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Human umbilical vein endothelial cell;
Metabolic memory;
NF-κB
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(5):432-434
- CountryChina
- Language:Chinese
-
Abstract:
Cultured human umbilical vein endothelial cells (HUVECs) were divided and assigned to 6 groups:normal control group,mannitol control group,high glucose group,low dose resveratrol group,medium dose resveratrol group,and high dose resveratrol group.HUVECs were pretreated with or without 30 mmol/L glucose plus various concentrations of resveratrol (0.1,1,10 μmol/L) for 16 hours and then incubated with 5.5 mmol/L glucose for 6 days.The levels of vascular cell adhesion molecule 1 (VCAM-1),monocyte chemotactic protein 1 (MCP-1),and plasminogen activator inhibitor 1 (PAI-1) in the culture supernatant were measured by ELISA.NF-κB expression was detected by Western blot.Compared with normal glucose group,high glucose up-regulated the expression of NF-κB,along with the increased levels of VCAM-1,MCP-1,and PAI-1 (all P＜0.05),which were still maintained at high levels even after withdrawal of high glucose.Resveratrol treatment down-regulated the expression of NF-κB and lowered the levels of VCAM-1,MCP-1,and PAI-1 (all P＜0.05).These results suggest that resveratrol may decrease the secretion of VCAM-1,MCP-1,and PAI-1 and prevent high glucose memory-induced injury to endothelial cell via NF-κB pathway.
